| 报告题目：Process Modeling and Cell Damage Evaluation in Laser-Assisted Living Cell Direct Writing 报告人： Dr. Yong Huang, Department of Mechanical Engineering, 报告时间： 报告地点：浙江大学玉泉校区流体传动及控制国家重点实验室四楼会议室 报告人简介: Dr. Yong Huang is an associate professor of Mechanical Engineering at |
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| 报告摘要: Process Modeling and Cell Damage Evaluation in Laser-Assisted Living Cell Direct Writing Yong Huang, Department of Mechanical Engineering, Maskless jet-based (including laser- and ink-based) cell direct writing is a revolutionary advance to print arbitrary cell patterns as well as to create heterogeneous three-dimensional living scaffolds cell-by-cell. Most importantly, cell direct writing provides a promising solution to current organ donor shortages by placing different cells to mimic the cellular organization of native organs, resulting in what is known as organ printing. So far, cell direct-write process-induced thermomechanical damage to cells as well as other biomaterials still poses a significant challenge to ensuring a satisfactory post-transfer cell viability. As previous studies show, thermomechanical loading can dramatically increase cell mortality rates during the cell droplet formation and landing processes if direct-write conditions are not properly selected. Using a representative laser-assisted cell direct-write technology (modified laser-induced forward transfer) as a model system, we have been addressing the aforementioned direct writing-induced cell damage challenge by studying 1) the process-induced cell thermomechanical loading profiles during cell droplet formation and landing processes; and 2) the post-transfer cell viability through understanding the mechanistic correlation between the cell damage/viability and process-induced thermomechanical loading profiles. Living cells are treated as a special workpiece material with unique material properties in this study. In this talk, modeling of the laser-induced bubble expansion and resultant cell mechanical loading during the cell droplet formation process will be first introduced. Then modeling of the cell droplet and hydrogel coating impact and resultant cell mechanical loading during the cell droplet landing process will be further discussed. Finally, the relationship between the post-transfer cell viability and the modeled mechanical loading information will be highlighted to evaluate the process-induced cell damage. It is found that the process-induced cell damage depends on not only the magnitudes of stress, acceleration, and/or shear strain but also the loading history that a cell experiences. It is expected that a complete understanding on manufacturing process-induced biomaterial damage in such jet-based direct writing will significantly promote safe implementation of biomaterial direct writing for biomedical research and manufacturing applications. 欢迎广大师生参加! |