报告人：Dr. Lingqian Chang (常凌乾), Department of Biomedical Engineering, The Ohio State University, USA
时间：4月19日，10:00 – 11:00
地点：教3 - 406
摘要：
Electroporation is one of the most commonly used physical techniques for gene /drug delivery. However, conventional bulk electroporation (BEP) usually results in significantly cellular damage, low efficiency and total stochastic transfection yields at the single-cell level. Recent advances in nanofabrication enable powerful nanoscale electroporation devices that offered remarkable advantages over BEPs. This talk presents a novel nanochannel-based electroporation technique that offers unique performance in benign and precise gene delivery into living cells both in vitro and in vivo. The challenges, designs and fabrication strategies behind the nanochannel devices are discussed in details. A versatile nanoscale platform, in combination of cellular manipulation technologies, is implemented to position, pattern and genetic manipulation of living cells. This high-throughput nano-electroporation systems show great potentials in deterministic cell reprogramming, efficient adoptive immunotherapy and intracellular sensing of living cancer stem cells based on molecular beacon/siRNA/mRNA delivery.
